Journal: Vaccines

Article Title: Recombinant BCG Expressing HTI Prime and Recombinant ChAdOx1 Boost Is Safe and Elicits HIV-1-Specific T-Cell Responses in BALB/c Mice

doi: 10.3390/vaccines7030078

Figure Lengend Snippet: Genetic characterization of the BCG.HTI 2auxo.int strain. ( A ) Pasteur substrain identification of BCG.HTI 2auxo.int strains by multiplex PCR assay. Lane 1: BCG.HTI 2auxoint Working Vaccine Stock giving the bands of 472 bp, 401 bp, 276 bp, and 196 bp; lane 2: BCG Connaught giving the bands of 401 bp, 252 bp, and 196/199 bp; lane 3 BCG Danish with bands of 472 bp, 401 bp, 276 bp, 252 bp, and 196 bp, lane 4: BCG Pasteur with bands of 472 bp, 401 bp, 276 bp, and 196 bp; lane 5: negative control, distilled water; and lane 6: Molecular weight marker. ( B ) Enzymatic restriction analysis of p2auxo.HTI int plasmid DNA purified from transformed E. coli M15∆glyA cultures (pre-BCG transformation). Lane 1: molecular weight marker; Lane 2: Apa1 digestion of p2auxo.HTI.int; Lane 3: BamHI digestion of p2auxo.HTI int ; Lane 4: Bsal digestion of p2auxo.HTI int ; Lane 5: StuI digestion of p2auxo.HTI.int; Lane 6: BamHI digestion of p2auxo.empty int ; Lane 7: NotI digestion of p2auxo.empty int . ( C ) PCR detection of the HTI gene in the BCG.HTI 2auxo.int WVS; lane 1: H 2 O; lane 2: p2auxo.HTI int plasmid; lane 3: working vaccine stock of BCG.HTI 2auxo.int ; lane 4: BCG wt; lane 5: BCG transformed with empty 2auxo.int plasmid; lane 6: molecular weight marker. (D) PCR detection of the GlyA gene in the BCG.HTI 2auxo.int working vaccine stock; lane 1: p2auxo.HTI int plasmid; lane 2: WVS of BCG.HTI 2auxo.int ; lane 3: BCGwt; lane 4: BCG transformed with empty 2auxo.int plasmid; lane 5: water; lane 6: molecular weight marker. PCR for right integration site, attR ( E,F ) left integration site, attL in the BCG.HTI 2auxo.int WVS; lane 1 WVS of BCG.HTI 2auxo.int ; lane 2: BCGwt; lane 3: distilled water; lane 4: molecular weight marker; lane 5: positive control, BCG.HIVconsv1 2auxo.int Working Vaccine Stock (recombinant BCG expressing the HIVconsv1 immunogen [ , ]).

Article Snippet: The HTI G+C rich DNA sequence was synthesized by Geneart (USA) and ligated to the integrative p2auxo.int plasmid fused to the 19-kDa lipoprotein secretion signal sequence generating p2auxo.HTI int .

Techniques: Multiplex Assay, Negative Control, Molecular Weight, Marker, Plasmid Preparation, Purification, Transformation Assay, Positive Control, Recombinant, Expressing

Journal: Cancers

Article Title: Single-Nucleotide Polymorphisms of BRCA1 and BRCA2 and Risk of Papillary Thyroid Carcinoma

doi: 10.3390/cancers17091456

Figure Lengend Snippet: ( A ) Schematic of the BRCA1 DNA repair-associated ( BRCA1 ) gene on chromosome 17q21.31. ( B ) Physical map of BRCA2 DNA repair-associated ( BRCA2 ) gene on 13q13.1.

Article Snippet: , rs1799966 , C_2615208_20.

Techniques:

Journal: Cancers

Article Title: Single-Nucleotide Polymorphisms of BRCA1 and BRCA2 and Risk of Papillary Thyroid Carcinoma

doi: 10.3390/cancers17091456

Figure Lengend Snippet: ( A ) Linkage disequilibrium (LD) of single-nucleotide polymorphisms of BRCA1. ( B ) LD of single-nucleotide polymorphisms of BRCA2. The LD was based on the genotypes of the Korean population. In the Haploview program, the D value is expressed as a value between 0 and 100. The value 98 means 0.98. A red diamond without a number represents 1.

Article Snippet: , rs1799966 , C_2615208_20.

Techniques:

Journal: Cancers

Article Title: Single-Nucleotide Polymorphisms of BRCA1 and BRCA2 and Risk of Papillary Thyroid Carcinoma

doi: 10.3390/cancers17091456

Figure Lengend Snippet: The association results of BRCA1 variants and the risk of PTC.

Article Snippet: , rs1799966 , C_2615208_20.

Techniques: